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Molecular basis of intramolecular electron transfer in sulfite-oxidizing enzymes is revealed by high resolution structure of a heterodimeric complex of the catalytic molybdopterin subunit and a c-type cytochrome subunit

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The samples were precipitated again by methanol-chloroform-water and then resuspended with 1 Laemmli buffer without DTT, boiled at 95 C for 3 min, separated by SDS-PAGE, and analyzed by immunoblotting

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This makes L-carnitine a conditionally essential nutrient